Obtaining Eggs from Xenopus laevis Females

Twin Xenopus laevis embryos appearing from flattened eggs

Remarkable progress has recently been made in molecular biology of double axis formation in Xenopus laevis. Leaving aside, for the time being, the problem of the gene expressions regulating Xenopus laevis development, here I show that pulse treatment could induce formation of a secondary axis in a fertilized Xenopus laevis egg. At 3 min after insemination, metal oxides were added to Xenopus fer...

ترتیب اعصای جانبی در دم لارو of Xenopus laevis

The S362A mutation block ROMK2 (Kir1.1b) endocytosis in Xenopus laevis oocyte membrane .

Abstract The S362A mutation block ROMK2 (Kir1.1b) endocytosis in Xenopus laevis oocyte membrane . Saeed Hajihashemi1 , 1-Assistant professor, PhD in Physiology, Department of Physiology, School of Medical science, Arak University of Medical Sciences. Introduction: ROMK channel is localized on the apical membrane of the nephron. Recent studies suggest that endocytosis of ROMK chan...

Diploid gynogenesis in Xenopus laevis and the localization with respect to the centromere of the gene for periodic albinism

Diploid gynogenetic Xenopus laevis were obtained by inseminating the eggs with u.v. irradiated spermatozoa, and treating them with hydrostatic pressure to inhibit the expulsion of the second polar body. A u.v. dose of 3000 ergs/mm genetically inactivates the spermatozoa without loss of their ability to activate egg development. The use of a genetic marker on very large samples of eggs made it p...

Expression of hsp90 Alpha and hsp90 Beta during Xenopus laevis Embryonic Development

Background: Members of the eukaryotic Hsp90 family function as important molecular chaperones in the assembly, folding and activation of cellular signaling in development. Two hsp90 genes, hsp90 alpha and hsp90 beta, have been identified in fish and homeothermic vertebrates but not in poikilothermic vertebrates. In the present study, the expression of hsp90 alpha and hsp90 beta genes in Xenopus...